Acta Anatomica Sinica

›› 2011, Vol. 42 ›› Issue (3): 394-398.doi: 10.3969/j.issn.0529-1356.2011.03.020

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Effect of Heshouwuyin on the expression of 3β-hydroxysteroid dehydrogenase protein in testis tissue of overtrained rats

  

  1. 1.Department of Basic Medical Science, Chengde Medical College, Hebei Chengde 067000, China; 2.Section of Histology and Embryology, Medical Department of Hebei United University, Hebei Tangshan 063009, China；3. Department of Histology and Embryology, Hebei Medical University, Shijiazhuang 050017, China; 4. Section of Histology and Embryology, Medical Department of Hebei University, Hebei Baoding 071000, China; 5. Graduates School of Hebei Medical University, Shijiazhuang 050017, China;6.Department of Gynaecology and Obstetrics, 260 Hospital of Peoples Liberation Army, Shijiazhuang 050041, China; 7. Hebei Normal University, Shijia zhuang 050016, China
  • Received:2010-12-24 Revised:2011-01-23 Online:2011-06-06
  • Contact: O Fu-lu

Abstract: Objective To explore the effect of Heshouwuyin(HSWY) on the expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) protein in testis tissue of overtrained rats. Methods Thirty SD rats were randomized into 5 groups, including normal control group(A group), HSWY administered normal group(B group), model control group(C group), HSWY treated group (D group), HSWY beforehand-prevented group (E group), 6 animals in each group. The model of C, D and E group were made by overtraining, the D group was treated with HSWY by intragastric administration for 60 days after the success of model［20g/(kgI>&#/I>8226;d) including crude drug 9.6kg/L］, and the E group was prevented by administering HSWY before overtraining［20g/(kgI>&#/I>8226;d) including crude drug 96kg/L］. The density of testosterone was detected by radio-immunity, the expression of 3β-HSD was measured by immunohistochemistry, Western blotting and RT-PCR. Results Compared with C group, 3β-HSD protein was expressed in plasmid of leydig cells, and the expressions of 3β-HSD and 3β-HSD mRNA in D group and E group were increased(EM>P/EM>0.05) with no differenc

Key words: Heshouwuyin（HSWY）, Overtraining, Testosterone, 3β-Hydroxysteroid dehydrogenase, RT-PCR, Western blotting, Immunohistochemistry, Rat

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